10 sanger box 做WGCNA,为什么不成功,哪里出了问题?

运行结果如下,图一和图二是软件界面,图三是结果界面

读取文件完毕

开始运行

文本处理完毕

C:\R\R-3.3.3\bin\Rscript.exe

C:\Users\A\Desktop\Sanger_V1.0.8\source\softs\2018-08-24-09-12-06-5b7f5b666949f\WGCNA_Pipline.R

开始运行R

"C:\R\R-3.3.3\bin\Rscript.exe" "C:\Users\A\Desktop\Sanger_V1.0.8\source\softs\2018-08-24-09-12-06-5b7f5b666949f\WGCNA_Pipline.R" "C:\Users\A\Desktop\WCGNA" "success_diffmRNAExp.txt" "success_TEST.txt" "1.2" "30" "unsigned" "100" "0.25" "0.9" "2" "0.02" "-1" "1"

==========================================================================

*

*  Package WGCNA 1.63 loaded.

*

*    Important note: It appears that your system supports multi-threading,

*    but it is not enabled within WGCNA in R. 

*    To allow multi-threading within WGCNA with all available cores, use 

*

*          allowWGCNAThreads()

*

*    within R. Use disableWGCNAThreads() to disable threading if necessary.

*    Alternatively, set the following environment variable on your system:

*

*          ALLOW_WGCNA_THREADS=

*

*    for example 

*

*          ALLOW_WGCNA_THREADS=8

*

*    To set the environment variable in linux bash shell, type 

*

*           export ALLOW_WGCNA_THREADS=8

*

*     before running R. Other operating systems or shells will

*     have a similar command to achieve the same aim.

*

==========================================================================



[1] "data.frame"

 Flagging genes and samples with too many missing values...

  ..step 1

ERROR : datExpr must contain numeric data. 

导出成功

attachments-2018-11-UJaIjrGZ5bda589e4e7d3.jpgattachments-2018-11-LHCooeyr5bda58a92d850.jpgattachments-2018-11-2ehbnbhv5bda58b0b28d4.jpg


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1 个回答

调研图

从报错来看是表达矩阵的数据类型不对,你重新保存一份数据看看,用execel另存为文本文件(Tab分割)

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