3 使用silva123数据库 qiime 1.7.0分析,filter_alignment.py 的-e -g如何设置

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liuzhe

filter_alignment.py -i pynast_aligned/otus_aligned.fasta -o pynast_aligned/ -e 0.10 -g 0.80       #(assume -e will suppresss default GG lane mask)


Those settings should be fine (the settings will be different on versions of QIIME earlier than 1.9.0, if so, use a value -e 0.0005, due to changes in the order of filtering).

Since the script will be finding the most entropic/gapped positions, it will be fine for 16S as well as 18S or mixed 18S/16S alignments.

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  • 王芳 提出于 2017-07-05 09:47

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