各位大神好,我在测序单细胞转录组可变剪切分析软件Outrigger时,在index之后进行validate,但是总是出现错误:
File "/hwfssz1/ST_MCHRI/DISEASE/USER/maoyong/software/anaconda3/envs/outrigger-env/bin/outrigger", line 6, in <module>
sys.exit(outrigger.commandline.main())
File "/hwfssz1/ST_MCHRI/DISEASE/USER/maoyong/software/anaconda3/envs/outrigger-env/lib/python3.5/site-packages/outrigger/commandline.py", line 1071, in main
cl = CommandLine(sys.argv[1:])
File "/hwfssz1/ST_MCHRI/DISEASE/USER/maoyong/software/anaconda3/envs/outrigger-env/lib/python3.5/site-packages/outrigger/commandline.py", line 344, in __init__
self.args.func()
File "/hwfssz1/ST_MCHRI/DISEASE/USER/maoyong/software/anaconda3/envs/outrigger-env/lib/python3.5/site-packages/outrigger/commandline.py", line 352, in validate
validate.execute()
File "/hwfssz1/ST_MCHRI/DISEASE/USER/maoyong/software/anaconda3/envs/outrigger-env/lib/python3.5/site-packages/outrigger/commandline.py", line 834, in execute
exonA, exonB, splice_abbrev)
File "/hwfssz1/ST_MCHRI/DISEASE/USER/maoyong/software/anaconda3/envs/outrigger-env/lib/python3.5/site-packages/outrigger/commandline.py", line 787, in exon_pair_splice_sites
exonA, splice_abbrev, 'downstream')
File "/hwfssz1/ST_MCHRI/DISEASE/USER/maoyong/software/anaconda3/envs/outrigger-env/lib/python3.5/site-packages/outrigger/commandline.py", line 800, in individual_exon_splice_sites
exon_bed, self.genome, self.fasta, direction)
File "/hwfssz1/ST_MCHRI/DISEASE/USER/maoyong/software/anaconda3/envs/outrigger-env/lib/python3.5/site-packages/outrigger/validate/check_splice_sites.py", line 57, in read_splice_sites
flanked = bed.flank(l=left, r=right, s=True, genome=genome)
File "/hwfssz1/ST_MCHRI/DISEASE/USER/maoyong/software/anaconda3/envs/outrigger-env/lib/python3.5/site-packages/pybedtools/bedtool.py", line 806, in decorated
result = method(self, *args, **kwargs)
File "/hwfssz1/ST_MCHRI/DISEASE/USER/maoyong/software/anaconda3/envs/outrigger-env/lib/python3.5/site-packages/pybedtools/bedtool.py", line 204, in not_implemented_func
raise NotImplementedError(help_str)
NotImplementedError:
"flankBed" does not appear to be installed
or on the path, so this method is disabled. Please install a more recent
version of BEDTools and re-import to use this method.
我是按照给的例子进行操作的,感到十分不解。
请问这是什么原因呢?