运用DEcenter的DESeq2进行分析总是报错,求解答。

之前还用了R3.3.1版本和3.5.0版本都是同样问题,都是出现没有这个DESeqDataSetFromMatrix函数,也看了祝老师之前回答的类似的问题,但还是没解决,运行edgeR就没有出现错误,求解答,非常感谢!



样本共分有2组

tumor组样本共有58个

normal组样本共有58个

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运行R命令:"C:\Program Files\R\R-3.4.2\bin\x64\Rscript.exe" "D:\dec_tmp_bak\limma.R" "D:\dec_tmp_bak" DESeq2 matrix.xls sample.xls 1 0.05

载入需要的程辑包:DESeq2

载入需要的程辑包:S4Vectors

载入需要的程辑包:methods

载入需要的程辑包:stats4

载入需要的程辑包:BiocGenerics

载入需要的程辑包:parallel


载入程辑包:'BiocGenerics'


The following objects are masked from 'package:parallel':


    clusterApply, clusterApplyLB, clusterCall, clusterEvalQ,

    clusterExport, clusterMap, parApply, parCapply, parLapply,

    parLapplyLB, parRapply, parSapply, parSapplyLB


The following objects are masked from 'package:stats':


    IQR, mad, sd, var, xtabs


The following objects are masked from 'package:base':


    anyDuplicated, append, as.data.frame, cbind, colMeans, colnames,

    colSums, do.call, duplicated, eval, evalq, Filter, Find, get, grep,

    grepl, intersect, is.unsorted, lapply, lengths, Map, mapply, match,

    mget, order, paste, pmax, pmax.int, pmin, pmin.int, Position, rank,

    rbind, Reduce, rowMeans, rownames, rowSums, sapply, setdiff, sort,

    table, tapply, union, unique, unsplit, which, which.max, which.min



载入程辑包:'S4Vectors'


The following object is masked from 'package:base':


    expand.grid


载入需要的程辑包:IRanges

载入需要的程辑包:GenomicRanges

载入需要的程辑包:GenomeInfoDb

Error: package or namespace load failed for 'GenomeInfoDb' in loadNamespace(j <- i[[1L]], c(lib.loc, .libPaths()), versionCheck = vI[[j]]):

 不存在叫'RCurl'这个名字的程辑包

Failed with error:  '无法载入程辑包'GenomeInfoDb''

此外: Warning message:

程辑包'GenomicRanges'是用R版本3.4.4 来建造的 

Bioconductor version 3.6 (BiocInstaller 1.28.0), ?biocLite for help

A new version of Bioconductor is available after installing the most recent

  version of R; see http://bioconductor.org/install

BioC_mirror: https://bioconductor.org

Using Bioconductor 3.6 (BiocInstaller 1.28.0), R 3.4.2 (2017-09-28).

Installing package(s) 'DESeq2'

试开URL’https://bioconductor.org/packages/3.6/bioc/bin/windows/contrib/3.4/DESeq2_1.18.1.zip'

Content type 'application/zip' length 4542389 bytes (4.3 MB)

==================================================

downloaded 4.3 MB


Old packages: 'stringi'

载入需要的程辑包:DESeq2

载入需要的程辑包:GenomicRanges

载入需要的程辑包:GenomeInfoDb

Error: package or namespace load failed for 'GenomeInfoDb' in loadNamespace(j <- i[[1L]], c(lib.loc, .libPaths()), versionCheck = vI[[j]]):

 不存在叫'RCurl'这个名字的程辑包

Failed with error:  '无法载入程辑包'GenomeInfoDb''

此外: Warning message:

程辑包'GenomicRanges'是用R版本3.4.4 来建造的 

package 'DESeq2' successfully unpacked and MD5 sums checked


The downloaded binary packages are in

C:\Users\Administrator\AppData\Local\Temp\RtmpOOhd4K\downloaded_packages

ERROR : 没有"DESeqDataSetFromMatrix"这个函数 


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