之前还用了R3.3.1版本和3.5.0版本都是同样问题,都是出现没有这个DESeqDataSetFromMatrix函数,也看了祝老师之前回答的类似的问题,但还是没解决,运行edgeR就没有出现错误,求解答,非常感谢!
样本共分有2组
tumor组样本共有58个
normal组样本共有58个
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运行R命令:"C:\Program Files\R\R-3.4.2\bin\x64\Rscript.exe" "D:\dec_tmp_bak\limma.R" "D:\dec_tmp_bak" DESeq2 matrix.xls sample.xls 1 0.05
载入需要的程辑包:DESeq2
载入需要的程辑包:S4Vectors
载入需要的程辑包:methods
载入需要的程辑包:stats4
载入需要的程辑包:BiocGenerics
载入需要的程辑包:parallel
载入程辑包:'BiocGenerics'
The following objects are masked from 'package:parallel':
clusterApply, clusterApplyLB, clusterCall, clusterEvalQ,
clusterExport, clusterMap, parApply, parCapply, parLapply,
parLapplyLB, parRapply, parSapply, parSapplyLB
The following objects are masked from 'package:stats':
IQR, mad, sd, var, xtabs
The following objects are masked from 'package:base':
anyDuplicated, append, as.data.frame, cbind, colMeans, colnames,
colSums, do.call, duplicated, eval, evalq, Filter, Find, get, grep,
grepl, intersect, is.unsorted, lapply, lengths, Map, mapply, match,
mget, order, paste, pmax, pmax.int, pmin, pmin.int, Position, rank,
rbind, Reduce, rowMeans, rownames, rowSums, sapply, setdiff, sort,
table, tapply, union, unique, unsplit, which, which.max, which.min
载入程辑包:'S4Vectors'
The following object is masked from 'package:base':
expand.grid
载入需要的程辑包:IRanges
载入需要的程辑包:GenomicRanges
载入需要的程辑包:GenomeInfoDb
Error: package or namespace load failed for 'GenomeInfoDb' in loadNamespace(j <- i[[1L]], c(lib.loc, .libPaths()), versionCheck = vI[[j]]):
不存在叫'RCurl'这个名字的程辑包
Failed with error: '无法载入程辑包'GenomeInfoDb''
此外: Warning message:
程辑包'GenomicRanges'是用R版本3.4.4 来建造的
Bioconductor version 3.6 (BiocInstaller 1.28.0), ?biocLite for help
A new version of Bioconductor is available after installing the most recent
version of R; see http://bioconductor.org/install
BioC_mirror: https://bioconductor.org
Using Bioconductor 3.6 (BiocInstaller 1.28.0), R 3.4.2 (2017-09-28).
Installing package(s) 'DESeq2'
试开URL’https://bioconductor.org/packages/3.6/bioc/bin/windows/contrib/3.4/DESeq2_1.18.1.zip'
Content type 'application/zip' length 4542389 bytes (4.3 MB)
==================================================
downloaded 4.3 MB
Old packages: 'stringi'
载入需要的程辑包:DESeq2
载入需要的程辑包:GenomicRanges
载入需要的程辑包:GenomeInfoDb
Error: package or namespace load failed for 'GenomeInfoDb' in loadNamespace(j <- i[[1L]], c(lib.loc, .libPaths()), versionCheck = vI[[j]]):
不存在叫'RCurl'这个名字的程辑包
Failed with error: '无法载入程辑包'GenomeInfoDb''
此外: Warning message:
程辑包'GenomicRanges'是用R版本3.4.4 来建造的
package 'DESeq2' successfully unpacked and MD5 sums checked
The downloaded binary packages are in
C:\Users\Administrator\AppData\Local\Temp\RtmpOOhd4K\downloaded_packages
ERROR : 没有"DESeqDataSetFromMatrix"这个函数