用的64位工具盒，下载TCGA RNAseq counts数据，根据临床数据分组，想做差异分析。昨天试用DEseq运行了1个多小时抱失败，今天使用edgeR包，结果如下：

运行R命令："F:\Research\sangerbox/source/softs/R-Portable/App/R-Portable/bin/Rscript.exe" "F:\dec_tmp_bak\limma.R" "F:\dec_tmp_bak" edgeR matrix.xls sample.xls 1 0.05
载入需要的程辑包：edgeR
Bioconductor version 3.5 (BiocInstaller 1.26.1), ?biocLite for help
A newer version of Bioconductor is available for this version of R,
  ?BiocUpgrade for help
BioC_mirror: https://bioconductor.org
Using Bioconductor 3.5 (BiocInstaller 1.26.1), R 3.4.1 (2017-06-30).
Installing package(s) 'edgeR'
also installing the dependency 'locfit'

试开URL’https://cran.rstudio.com/bin/windows/contrib/3.4/locfit_1.5-9.1.zip'
Content type 'application/zip' length 594554 bytes (580 KB)
==================================================
downloaded 580 KB

试开URL’https://bioconductor.org/packages/3.5/bioc/bin/windows/contrib/3.4/edgeR_3.18.1.zip'
Content type 'application/zip' length 4343680 bytes (4.1 MB)
==================================================
downloaded 4.1 MB

Old packages: 'cluster', 'foreign', 'lattice', 'limma', 'MASS', 'Matrix',
  'mgcv', 'nlme', 'rpart', 'survival'
载入需要的程辑包：edgeR
载入需要的程辑包：limma
Warning messages:
1: In library(package, lib.loc = lib.loc, character.only = TRUE, logical.return = TRUE,  :
  不存在叫'edgeR'这个名字的程辑包
2: In file(file, ifelse(append, "a", "w")) :
  无法打开文件'Intermediate/Normal-vs-Favorable.edgeR.txt': No such file or directory
package 'locfit' successfully unpacked and MD5 sums checked
package 'edgeR' successfully unpacked and MD5 sums checked

The downloaded binary packages are in
 C:\Users\sty\AppData\Local\Temp\Rtmpsnth8w\downloaded_packages
ERROR : 无法打开链结